Basic Local Alignment Search Tool - BLAST
Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence …
Primer-Blast results - National Center for Biotechnology Information
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.
Primer-BLAST: A tool to design target-specific primers for …
Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on …
Electronic-PCR (e-PCR) is retiring, use Primer-BLAST instead
Jun 28, 2017 · The good news is that an existing tool, Primer-BLAST, fills in nicely for the functions of both Forward and Reverse e-PCR, and has the additional benefit of de novo …
Molecular basis for the initiation of DNA primer synthesis
The structural and mechanistic studies presented here establish how Prim-Pol proteins instigate primer synthesis, revealing the requisite molecular determinants for primer synthesis within the …
PrimerBank: a resource of human and mouse PCR primer pairs for …
For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus …
Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions. It uses these to limit the locations of primers. The longest …
Neuropathic pain - A clinical primer - PubMed
Nov 24, 2024 · Neuropathic pain is used both as a mechanistic descriptor and a classification category of pain caused by a lesion or disease of the somatosensory nervous system and …
Parameters for Successful PCR Primer Design - PubMed
Despite the publication of numerous guidelines, the actual design of many published assays is often unsound: primers lack the claimed specificity, they may have to compete with secondary …
Primer-BLAST now designs primers for a group of related sequences
Sep 25, 2020 · To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions. It uses these to limit the locations of primers.