Cryo-EM performance is fundamentally constrained not only by technical preparation challenges but also by intrinsic protein disorder, where dynamic and flexible regions remain unresolved despite ...

P-type ATPases play essential roles in maintaining ion homeostasis across cellular membranes, yet structural studies of these dynamic membrane proteins are often limited by sample heterogeneity, ...

Cryo-electron microscopy (cryo-EM) can help scientists determine the three-dimensional structure of proteins in unprecedented detail. Jacques Dubochet, former group leader at EMBL, shared the 2017 ...

Sample loss has been a persistent problem in cryo-EM, a high-tech method for creating 3D models of molecules that reveal their inner structures. It occurs during an essential step of imaging ...

Support grids are a key part of electron microscopy measurements; the choice of the grid can directly influence the quality and accuracy of the final image. This is particularly true for transmission ...

What is Cryo-Electron Microscopy? Cryo-electron microscopy (cryo-EM) is a powerful technique used to determine the three-dimensional structure of biological macromolecules, such as proteins and ...

Particles (encircled in green) are spaced at a distance from the noise surrounding the nanobeads (purple), which would otherwise obscure them. Sample loss has been a persistent problem in cryo-EM, a ...