This protocol outlines a procedure for collecting and analyzing point spread functions (PSFs). It describes how to prepare fluorescent microsphere samples, set up a confocal microscope to properly ...

(Nanowerk Spotlight) A conventional optical microscope equipped with an oil immersion objective can resolve objects no smaller than about 200 nanometers – a restriction known as the diffraction limit.

A Linnik interferometer microscope is designed to take high-resolution images of the surface topography of a sample. The device works by splitting a beam of illuminating light in two, with one beam ...

(Nanowerk News) Writing in the journal Nature Communications, the team have created a microscope which shatters the record for the smallest object the eye can see, beating the diffraction limit of ...

What is microsphere nano-imaging? A microsphere is a spherical particle between one and one thousand micrometers (μm) and in diameter. 1,000 μm is the same size as one millimeter, while 1 μm is 1,000 ...

The conventional microscopy is limited by the diffraction limit which is 250 nanometers. Thus, it is rather difficult to resolve objects that are smaller than that. However, some improvements have ...

Optical measurement techniques collecting light intensity in the far-field such as conventional and confocal microscopy or coherence scanning interferometry (CSI) enable fast and contactless ...